The discovery, characterization, and the application of the CRISPR/CAS system has revolutionized the study of genetics and genetic engineering. Please select all correct answers regarding its mechanism of action and application. Multiple correct answers are possible, and partial credit will be given.A. CRISPR sequences in bacteria resemble those from bacteriophage and plamid DNA.
B. For reverse genetics studies, CRISPR/CAS can be used to replace wildtype alleles with mutant alleles which can knockout the function of the target genes.
C. CRISPR is a DNA sequence in the bacterial chromosome.
D. The guide RNA directs CAS to specific DNA sequences.
E. The CRISPR/CAS system was discovered in bacteria, and its natural function is the equivalent of an immune system in bacteria for "remembering" viral infections and for attacking viral DNA in future infections.

Answers

Answer 1
Answer:

Answer:

A. CRISPR sequences in bacteria resemble those from bacteriophage and plasmid DNA. (The CRISPR sequences are derived from previously invading bacteriophages and pladmids.)

B. For reverse genetic studies, CRISPR /CAS can be used to replace wild type alleles with mutant alleles which can knock out the function of target genes. (CAS 9 introduces double strand break (DSB) in the target sequence which is then repaired by non homologous end joining (NHEJ). NHEJ causes deletion, insertion or frame shift mutation leading to knock out of the target genes.)

C. CRISPR is a bacterial sequence in the bacterial chromosome. ( The sequences are derived from bacteriophages that had infected the bacteria defore.)

D. The guide RNA guides CAS to specific DNA sequences. ( The guide RNA directs CAS nuclease to the target sequences for editing.)

E. The CRISPR/CAS system was discovered in bacteria, and it's natural function is the equivalent of an immune system in bacteria for remembering viral infections and for attacking viral DNA in future infections. (The CRISPR/CAS system provides acquired immunity to bacteria.)

Explanation:

see answer


Related Questions

I did the lab but I don’t know what goes in the table.
Science In a cell, what is the function of the cell membrane?OA. It only maintains the cell shape.ОВ.It removes waste and stores ingested food.OC. It generates energy for the cell.OD.It controls the entry and exit of substances.Have a good day ❤️
Landforms is a physical feature on earth's surface true or false
When sugars are broken down to produce usable energy for cells, a large amount of heat is released. Explain how the water inside a cell helps to keep a cell’s temperature constant.
What comments do these authors make concerning the relation of bases on one strand of the helix as compared to those on the other strand?

Which is one factor of rock types that affects the rate of weathering?

Answers

Answer:

porosity,minerals of softness,ease of dissolving

Explanation:

Answer:

Moisture B

Explanation:

At least I think, Dont be mad if you get it wrong tho LOL sorry guys.

Proteins accomplish many cellular tasks such as facilitating chemical reactions, providing structure, and carrying information from one cell to another. How a protein chain coils up and folds determines its three-dimensional shape. Its shape will, in turn, determine how it interacts with other molecules and thus performs its function in the cell.

Answers

Answer:

True.

Explanation:

Protein is a very versatile molecule and extremely important for the efficient functioning of the organism. However, proteins can only perform their functions through chains that wind and fold to form three-dimensional structures. these three-dimensional structures are the active form of the protein because they build the active site that will allow the substrate needed for body functions to fit and be catalyzed as needed.

In other words, the protein needs to be in its three-dimensional form for the construction of the active site to take place.

Which of the following statements can be attributed to water's versatility as a solvent

Answers

Answer:

The polar nature of water make water universal solvent.

Explanation:

The polar nature of water is responsible for the versatility as a solvent because due to this polar nature of water maximum number of solutes or chemicals dissolved in it and it is also called universal solvent. Polar nature means making positive and negative polar which attracts the opposite charge atoms and making covalent bond with them. The hydrogen has partial positive  charge so it attracts negative charge atom while oxygen has partial negative charge so it attracts positive charge atom.

Water's versatility as a solvent can be attributed to its polarity, hydrogen bonding, and high dielectric constant.

Water's versatility as a solvent can be attributed to several factors:

Polarity:

Water is a polar molecule, meaning it has a slightly positive end and a slightly negative end. This allows water to dissolve many different substances that have charged or polar molecules, like salts and sugars.

Hydrogen Bonding:

Water can form hydrogen bonds with other molecules. This allows it to dissolve substances that can form hydrogen bonds with water, such as alcohols and organic acids.

High Dielectric Constant:

The dielectric constant of water is relatively high, which means it can weaken intermolecular forces between solute particles and allow them to dissolve more easily.

For more such questions on Water's versatility, click on:

brainly.com/question/32911730

#SPJ6

The food web for a particular ecosystem is shown below.Greenhopper
Gras
Duck
Ant
Lizard
Frog
Tadpole
Snake
Sunlight
Algse
Bacteria
Decayed
Matter
Earthworm
What would most likely happen i the grasshopper population had a major increase?
A. The frog population would decrease.
B. The snake population would increase
c. The duck population would decrease,
D. The lizard population would not change.

Answers

Answer:

The snake population would increase.

Explanation:

Answer:

B

Explanation:

Just had the question

the snake does not get as much energy from eating the same amount of food as the grasshopper. The statement that best explains why this is true is

Answers

Answer:

Grasshoppers use up much of their energy for life processes.

Explanation:

Answer:

hm i have been wanting the answer to the same question

Explanation:

What is one difference between p-waves and s-waves

Answers

p waves travel through solid liquid or gas and s waves travel through solid but is stopped by liquid and gas.