Assume that you transform bacteria with a plasmid containing an ampicillin-resistance gene. Instead of directly plating the transformed population as you did in this lab, you set up two liquid cultures of them, one that contains ampicillin and one that does not. You will then assay these cultures on plates at two different times: immediately after you set up the cultures, and then again after the bacteria have been in culture for an extended period. The assays will demonstrate the number of ampicillin-resistant vs. ampicillin-sensitive culture bacteria in each culture at each time. To perform each of the two assays, you prepare serial dilutions of the two cultures and plate them onto LB plates with and without ampicillin (the dilution) is simply to ensure that you will get some plates on which you can distinguish separate colonies).Describe what you expect to observe in the initial assay and in the second assay. What, if any, differences might you expect in terms of the ratios of ampicillin-resistant and nonresistant bacteria?

Answers

Answer 1
Answer: In the initial assay with ampicillin, there will still be, although in a smaller number, nonresistant bacteria that have survived incubation. After an extended period, ampicillin will kill all nonresistant bacteria and in the second assay, only ampicillin-resistant bacteria will survive.

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Select an ecosystem that is familiar to you and describe the biotic and abiotic factors that exist there?

Answers

An ecosystem consist of various biotic and abiotic factors which interact with each other. A grassland ecosystem is a terrestrial ecosystem which have grass as the main producers (dominant) living thing.

What is a Grassland Ecosystem?

A grassland ecosystem is a type of terrestrial ecosystem in which grasses and the herbaceous plants are the dominant species. Grass controls the grassland ecosystems as they are the main producers of the ecosystem with few or no trees where there is not favorable conditions for a forest. It is more like a dry and desert ecosystem. Therefore, it is also called as a transitional landscape.

The four important abiotic components of grassland ecosystem includes climate, parent material and soil, topography, and natural disturbances. Climate includes the rainfall pattern, temperature and wind patterns of a particular area.

Biotic Factors in the Grassland ecosystem include Plants, Insects (Invertebrates), Grazers, and Predators.

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Answer:

Forest Ecosystem

An ecosystem is composed of two main components: biotic and abiotic factors. Biotic factors are the living parts of the ecosystem, such as plants, animals, insects, fungi, and bacteria. Abiotic factors are the non-living parts of the ecosystem, which influence the size and composition of the living parts: these are components like minerals, light, heat, rocks, and water.

Biotic Factors by Type

The most obvious features of any forest ecosystem are its trees, the dominant biotic feature. They dominate the ecosystem: both in terms of visibility and in terms of biomass. However, they are only one type of organism living in a forest. Other biotic factors include:

shrubs, flowering plants, ferns, mosses, lichens, fungi, mammals, birds, reptiles, insects, worms, microbes

Abiotic Factors by Type

The most important abiotic feature of a forest ecosystem may not be obvious, despite its ubiquity and importance: sunlight. Tangible abiotic factors include soil, minerals, rocks, and water. But abiotic factors can be intangible, such as temperature, other types of radiation, and the chemistry of soil and water.

Biotic Factors by Function

Ecologists frequently group an ecosystem's factors by what role they play in the system, rather than by what particular species they are. This is known as a functional classification.

These functions relate to the movement of energy through an ecosystem, and trees — along with other photosynthetic plants — are the chief primary producers. This means that trees convert the sun's energy into food energy, which is then used by other members of the ecosystem.

These other members of the ecosystem can also be categorized. Primary consumers are, for example, herbivores that eat the primary producers. Secondary consumers are the carnivores and omnivores that eat the primary producers. Decomposers are the scavengers, microbes, and fungi that consume the droppings and the carcasses of other organisms.

Abiotic Factors by Function

The abiotic factors of a forest fall less obviously into functional classifications, but keep in mind that the energy transferred among the various biotic categories is itself a foundational abiotic element. This energy occurs in the form of solar radiation, which includes both visible light and heat (infrared).

Primary producers (plants like trees and shrubs) convert the light into carbohydrates, a form of energy that can be consumed by other organisms. The function of other abiotic factors relies on the minerals they contain, such as the nitrogen in the soil or the hydrogen in water molecules.

What would be the best microscope to view living single-celled organisms in a sample of pond water? a. Transmission electron microscope
b. Scanning electron microscope
c. Compound light microscope
d. Dissecting microscope

Answers

C) Compound light microscope would be the best microscope to view living single-celled organisms in a sample of pond water.

Compound light microscope  would be the best microscope to view living single-celled organisms in a sample of pond water

Which is least likely to be a reservoir for fresh water

Answers

Oceans would least likely to be a reservoir for fresh water :)

If a personality survey with high internal consistency includes two different questions related to the personality dimension of agreeableness, then which of the following is true?A.It is impossible to predict how people will answer the second question based on how they answered the first question.

B.If people give an extreme answer to the first question, they will tend to give the opposite extreme answer to the second question.


C.People will tend to give dissimilar answers to both questions.

D.People will tend to give similar answers to both questions.

E.If people give a moderate answer to the first question, they will tend to give an extreme answer to the second question.

Answers

If a personality survey is conducted we can see that people will give two dissimilar answers.

Explanation:

If a survey based on personality is conducted and the people are asked two question which are different in nature. We can see that people answering both the question will have dissimilar answer because according to the personality of people two different question cannot have a same answer , they must be different , that is why there is a tendency to get two different answer from the survey.

Answer:

hui

Explanation:

sdfgsdfg

Samuel wants to plant cuttings from his rose plant. What should he do to hasten the rooting process?

Answers

Spray auxin at the base of the rose cutting. Hope that helps :)

the correct answer choice is "C"

Breathing in humans is driven by all of the following except A. the muscles attached to the ribs. B. the diaphragm. C. air pressure. D. the muscles connected to the lungs.

Answers

Air pressure, all of the others are found within the body and contribute to both the lungs and the heart.