What enzyme is used to make radioactive labeled DNA

Answers

Answer 1
Answer:

Answer:

Explanation:

DNA polymerase

Use of radioactive deoxyribonucleotides as raw material for DNA synthesis makes DNA polymerase to use these radioactively labeled dNTPs to make new DNA strands. Hence, DNA polymerase enzyme can make radioactively labeled DNA by using radioactive deoxyribonucleotides as raw material.


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Which graphic organizer would help you visualize the similarities and differences between whales and dolphins? herringbone organizer Venn diagram context chart

Answers

Answer:

Option C, Venn Diagram

Explanation:

Venn diagram is a mathematical logical relationship that represents the relationship ship between two or more set of data through intersecting circles. The intersection area between the circles represent the similarities between the different set of data while the non-intersecting area of the circle represent the individualistic characteristics of each data set.  

Hence,  the characteristics of whale and dolphin can be represented by two circles in which the intersecting area of the circle will contain the similarities between the whale and dolphins and the non-intersecting areas will contain their individual unique characteristics.

Venn diagram. Hope this helps!

1. Scientific theory
What is the definition of this answer ?

Answers

Answer:

scientific theory:  an explanation of an aspect of the natural world that can be repeatedly tested and proved to be a success every time.

N snails, the shell may either coil to the right or the left. coiling to the right is dominant. a homozygous recessive female snail is crossed with a homozygous dominant male snail. all of the offspring have shells that coil to the left. why do all of the offspring have left-coiled shells

Answers

So first off homozygous means that both alleles are the same
If coiling to the right is dominant and to the left is recessive let capital c (C) represent right coiling and let lower case c (c) represent left coiling.
The female who is homozygous recessive must have both recessive alleles
The male who is homozygous must have two dominant alleles
So the cross will be between a CC and a cc
So we set up a Punnett square

We take a look at all the combinations and arrange them


So we find that there is a 25% chance of the offspring having left coiling

The coiling direction of the snail shell is affected by the maternal genes. All the offspring have left-coiled shells, as the phenotype is determined by the mother's genotype. Thus, option C is correct.

What is maternal inheritance?

Maternal inheritance has been defined as the effect by which the genes are influenced by the mother's genotype and are a type of non-Mendelian inheritance.

The dextral and the sinistral coiling seen in the snails showing the maternal effect is influenced by the genotype of the mother and is not affected by the father's genotype.

The mother has the cc and the father has the CC genes that when crossed are shown in the image.

From the cross, it is evident that the genotype of the mother snail affects and influences the genotype of the offspring, and hence all the offspring's have the left coiling (sinistral).

Therefore, in option C, the genotype of the mother snail affects the coiling.

Learn more about maternal inheritance, here:

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Your question is incomplete, but most probably your full question was,

All of the offspring have shells that coil to the left. Why do all of the offspring have left-coiled shells?

A. This trait follows a typical Mendelian pattern of inheritance.

B. Only the father's genotype determines the phenotype for shell coiling.

C. Only the mother's genotype determines the phenotype for shell coiling.

D. Shell-coiling is a sex-linked trait.

which answer below best describes how crispr/cas9 is used to delete or ablate gene function in cells or an organism?

Answers

The answer that best describes how CRISPR/Cas9 is used to delete or ablate gene function in cells or an organism is:
CRISPR/Cas9 is a revolutionary gene editing tool that utilizes a small RNA molecule (guide RNA) and a protein (Cas9) to target and modify specific genes.

To delete or ablate gene function, the following steps are typically followed:

1. Designing the guide RNA: Scientists design a guide RNA that is complementary to the target gene sequence. This guide RNA helps Cas9 to locate and bind to the specific gene of interest.

2. Delivery of CRISPR/Cas9 components: The guide RNA and Cas9 protein are introduced into the cells or organism that needs gene modification. This can be done using different delivery methods, such as viral vectors or direct injection.

3. Cas9 binding and DNA cleavage: Once inside the cells, the guide RNA directs Cas9 to bind to the target gene. Cas9 then acts as molecular scissors, cutting the DNA at a specific location within the gene.



4. Repairing the DNA: The cell's natural DNA repair machinery kicks in to fix the broken DNA. In some cases, this repair process can introduce errors, leading to gene disruptions or inactivation.

5. Gene function deletion or ablation: The repaired DNA may contain insertions or deletions that disrupt the gene's function. These disruptions can prevent the gene from producing a functional protein, effectively deleting or ablating its function.

It's important to note that the specific details and techniques may vary depending on the experiment or application. CRISPR/Cas9 offers a powerful and versatile tool for targeted gene editing, opening up new possibilities for studying gene function and potentially treating genetic diseases.

To know more about    CRISPR/Cas9      visit:

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CRISPR/Cas9 is a revolutionary gene-editing tool that has revolutionized the field of genetic engineering. It allows scientists to precisely delete or ablate gene function in cells or organisms by targeting specific DNA sequences and introducing modifications.

The CRISPR/Cas9 system consists of two main components: the Cas9 protein and a guide RNA (gRNA). The Cas9 protein acts as a molecular scissors, while the gRNA serves as a guide to direct Cas9 to the desired target site in the genome.

The process begins with the design and synthesis of a specific gRNA that is complementary to the target DNA sequence. The gRNA is engineered to recognize and bind to a specific region adjacent to the target gene. Once bound, the Cas9 protein creates a double-stranded break (DSB) at the target site.

Upon creating the DSB, the cell's natural DNA repair mechanisms come into play. There are two primary repair pathways: non-homologous end joining (NHEJ) and homology-directed repair (HDR). NHEJ is an error-prone repair mechanism that often introduces small insertions or deletions (indels) at the site of the break, leading to frameshift mutations and gene disruption. On the other hand, HDR relies on a template DNA molecule to repair the DSB accurately.

To delete or ablate gene function, researchers typically exploit the error-prone NHEJ pathway. By introducing CRISPR/Cas9 components into cells or embryos, they can induce targeted DSBs near the gene of interest. The subsequent repair by NHEJ often results in small indels that disrupt the reading frame of the gene, leading to premature stop codons or non-functional proteins. This effectively knocks out or ablates gene function.

It is worth noting that while NHEJ-mediated indels are commonly used for gene knockout studies, they can also introduce unintended mutations or off-target effects. Therefore, it is crucial to carefully design and validate gRNAs to minimize off-target activity.

In addition to gene knockout, CRISPR/Cas9 can also be used for gene ablation through other mechanisms. For example, researchers can design gRNAs to target specific regulatory regions of a gene, such as promoters or enhancers, to disrupt its expression without altering the coding sequence. This approach allows for more precise control over gene function.

Overall, CRISPR/Cas9 has revolutionized the field of genetic engineering by providing a powerful and versatile tool for deleting or ablating gene function in cells or organisms. Its simplicity, efficiency, and precision have made it an invaluable tool for studying gene function, disease modeling, and potential therapeutic applications

Medical science provides many ways to diagnose the cause of infertility and to treat it. . . What is the procedure that involves “washing” to separate healthy sperm from the unhealthy ones, and then placing this concentration of healthy sperm into the uterus around the time of ovulation?.

Answers

The answer is Intrauterine insemination.

  • In thistechnique of interauterine insemination, the female is given ovulation stimulating hormone or medication and after this monitoring is done to determine when the eggs mature.
  • After ovulation, a semen sample is washed to separate the sperms from the seminal fluid( removing the unhealthy ones).
  • Then a catheter is used to transfer the sperm or to insert the sperm directly in the uterus.
  • This process increases the chances of conception.


Answer:

intrauterine insemination

Explanation:

quizlet said so

Class O stars are the: hottest coolest brightest dimmest

Answers

The hottest is the answer

Class O stars is the hottest type of star