You have a culture of yeast that is at a concentration of 6.74 x 10^6 cells/ml. You dilute the sample 1:100, and then 1:100 again, and finally you dilute the sample an additional 1:3. You add 0.1 ml of the final dilution to a spread plate. 1. Assuming that most of the cells in the original culture were living, how many CFUs do you expect to count on your spread plate the next day?

Answers

Answer 1
Answer:

Answer:

22 cells

Explanation:

The concentration of numbers of cells = 6.74 x 10⁶ cells/ml

Dilution stages includes =  1:100  ;     1:100    &        1:3

The consecutive dilution stages can be calculated as:

= (6.74 x 10⁶) × (1/100)(1/100)(1/3)

=  222.42 cells/ml

= 2.22 × 10² cells/ml

So after addition  0.1 ml of the final dilution to a spread plate, the number of CFUs ( Colony forming units) we expect to count will be:

= (2.22 × 10² cells/ml)(0.1 ml)

= 22.2 cells

≅ 22 cells.


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List two chemicals that you think have low entropy and two that have high entropy. Explain the reason for your choices.

Answers

The Two substance that has low entropy are diamond and graphite while the other two chemical/substance that has high entropy are Noble gases(helium, neon, etc) and oxygen.

Generally, entropy measures the disorder or randomness in a system. High entropy implies randomness or high disorder while low entropy indicates orderliness.High entropy system, more energy is lost to the environment/surroundings.

Examples of chemicals or substances with high and low entropy are listed below

Low entropy

1.Diamond: This is a carbon crystalline substance. The makeup follows a well-structured pattern.  The particles are tightly packed thereby giving them a low entropy value. This means they are well ordered.

2.Graphite: This is another carbon substance but they have higher entropy than diamond but generally possess a low entropy.

High entropy

Generally, gaseous substances possess high entropy, and this includes

1. Nobles gases(helium, argon, Neon, etc); These gases when placed in a closed system they exhibit high entropy. They act disorderly. They move randomly and tend to occupy every space available in the system.

2. Oxygen gas: This is another gaseous substance that exhibits disorderliness when placed in a closed system.  

The arrangement of the particles and cohesive forces holding the individual particles of the substance together play a large role in determining its entropy.

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Here are two examples:  

Low entropy: -A carbon crystal structure at a temperature near absolute zero,              

                      -A chunk of ice has low entropy

High entropy:- A box filled with two elements in their gaseous state, both of which are noble gases.

                       - Burning wood illustrates an increase in entropy  heated very          high temperature, with the gas "not very dense".

 The entropy of a chemical system depends on its energy and its multiplicity,  or how many different ways its atoms and molecules can be arranged. By adding new arrangements or energy, you increase entropy.  A diamond, for example, has low entropy because the crystal structure fixes its atoms in place. If you smash the diamond,  entropy increases because the original, single crystal becomes hundreds of tiny pieces that can be rearranged in many ways.

Burning wood illustrates an increase in entropy as The wood starts as a single, solid object.  Fire consumes the wood, releasing energy along with carbon dioxide and water vapor, and leaving a pile of ashes.  The atoms in the vapors and gases vibrate energetically, spreading out in an ever-expanding cloud.  Dissolving salt in water is another example of increasing entropy; the salt begins as fixed crystals,  and the water splits away the sodium and chlorine atoms in the salt into separate ions, moving freely with water molecules.  A chunk of ice has low entropy because its molecules are frozen in place.  Add heat energy and entropy increases. The ice turns to water, and its molecules agitate like popcorn in a popper.

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Answers

The stages of replication is  Attachment, Penetration and Replication

The following information should be considered:

  • A bacteriophage means the virus that attacks bacterial cells.
  • The lytic and lysogenic cycles comrpise of two methods of viral replication.
  • In the lytic cycle, the virions generated are released from the host cell while on the other hand in the lysogenic cycle, viral nucleic material are existed into host nucleic material and also they are copied to daughter cells at the time when the host cell reproduces.

The common steps in both cycles are given below:

  1. Attachment – in this step, the bacteriophage attaches itself to the host surface cell to insert its DNA into the host cell.
  2. Penetration – the virus inserts its DNA into the host cell via penetrating the cell membrane of the host cell.
  3. Replication – the viral nucleic material is replicated using the host cell's replication mechanism.

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Answer:

Attachment, Penetration and Replication

Explanation:

A bacteriophage is a virus that attacks bacterial cells. The lytic and lysogenic cycles are two methods of viral replication. In the lytic cycle, the virions produced are released from the host cell whereas in the lysogenic cycle, viral nucleic material are incorporated into host nucleic material and are copied to daughter cells when the host cell reproduces. The common steps in both cycles are given below:

1  Attachment – in this step, the bacteriophage attaches itself to the surface of the host cell so as to insert its DNA into the host cell.

2. Penetration – the virus inserts its DNA into the host cell by penetrating the cell membrane of the host cell.

3. Replication – the viral nucleic material is replicated using the host cell's replication mechanism.