You transform an E. coli bacterium with the pGLO plasmid. After growing the +pGLO bacteria on an LB+Ampicillin+Arabinose plate, you see distinct colonies form after 16 hours. You check these colonies under the UV light and see that they are glowing a bright fluorescent green color. If you use a sterile loop and inoculate a new plate with LB+Ampicillin+Arabinose (coated on them, as usual) with these transformed E. coli will you see any growth? If there is growth, will it glow?

Answers

Answer 1

Answer:

Options for the question have not been provided. The complete question has been attached.

Answer:

A. Yes, we will see growth. Yes, they will glow.

Explanation:

pGLO plasmid was engineered to be used as a vector in the field of biotechnology. It has an ampicillin resistance gene which acts as a reporter gene. It also has another reporter gene, GFP which shares its promoter (araC) with the gene responsible for metabolizing arabinose. GFP gene is thus translated only in the presence of arabinose in the medium since the promoter is active only in the presence of arabinose. Once the GFP gene is translated, the colonies glow with green fluorescence under UV light.

Here, the E.Coli colonies were grown successfully on LB + Ampicillin + Arabinose plate so they were ampicillin resistant. They also glowed in UV light. If these transformed colonies are inoculated in another LB + Ampicillin + Arabinose plate they will be able to grow successfully since they are ampicillin resistant. They will also glow because the new plate also has arabinose so the araC promoter will be active and expression of both GFP gene and arabinose metabolizing gene will occur.

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What is the shape of DNA?O Double helixO An X shapeO None of the aboveO Single stranded

What are physical characteristics that help an organism survive

Answers

Answer:

Special characteristics adapted by specific animals allow them to carry out unique abilities in order to survive. For instance, a cheetah's long tail helps it balance when running at rapid speed in order to hunt. Or an arctic woolly bear caterpillar is able to be frozen and fill it's body with an antifreeze hormone that allows it to survive temperatures below freezing.

A skin cell divides to make a new skin cell. Which component of cell theory does this best illustrate?All living things are made of cells.
Cells are the basic unit of structure and function of all living things.
All cells come from other cells.
Cells are generated from nonliving materials.

Answers

i think it’s all cells come from other cells, because the skin cell dividing is creating a new cell, which originated out of the original cell

Answer:

its C

Explanation:

all cells come from other cells

What does the cardiovascular system do?carries hormones for the endocrine system
transports oxygen and carbon dioxide for the respiratory system
sends nerve impulses to the brain and spinal cord
carries nutrients for the digestive system
works with the immune system to fight infection

Answers

Answer:

transports oxygen and carbon dioxide for the respiratory system

Explanation:

It’s could be the second one but I’m not sure sorry

Which of the following is the functional unit of a myofibril? a. sarcomere
b. myofilament
c. sarcolemma
d. endomysium

Answers

Answer:

Sarcomere

Explanation:

Myofibril is the unit of the muscle cell. The myofibril is rod like in structure and developed by the process of myogenesis in embryo.

Sarcomere is the basic structural unit of the skeletal muscles and shows striations under the microscope. Sarcomere consists of thick and thin filaments of actin and myosin. Different bands like A- band , H-Band, I-Band and Z- line is visible in the sarcomere.

Thus, the correct answer is option (A).

A virus requires a host cell in order to?