Ten grams of hamburger were added to 90 ml of sterile buffer.This was mixed well in a blender. One-tenth of a ml of this slurry
was added to 9.9 ml of sterile buffer. After thorough mixing, this
suspension was further diluted by a 1/100 dilution followed by a
1/10 dilution. One-tenth of a ml of this final dilution was plated
on agar plates. After incubation, 52 colonies were present. How
many colony-forming units were present in the total 10 gram sample
of hamburger?

Answers

Answer 1
Answer:

Answer:

5.2 × 10 ⁹ cfus

Explanation:

Using the dilution factors

0.1 ml of the final dilution has 52 colonies

1 ml will have approximately 520 colonies

10 ml of the final sample will have 5200 colonies

at 1 / 100 dilution

1 ml of the sample will have 5200 colonies

100 ml of the sample will have 520000 colonies

1 ml of the 0.1 ml + 9.9 ml has 520000 colonies

10 ml will have   5200000

at the second stage of the dilution

0.1 ml of the slurry had 5200000 colonies

1 ml will have 52000000 colonies

10 ml will have 520000000 colonies

100 ml of the initial sample ( 10 grams + 90 ml ) = 5200000000 colonies =

5.2 × 10 ⁹ cfu


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Answers

During an enzymatic reaction, their is an decrease in the concentration of substrate, an increase in the concentration of product, and no change in the concentration of the enzyme.

What is enzymatic reaction?

A reaction, which is mediated with the help of an enzyme is termed as enzymatic reaction. A substance, which functions as a catalyst in living organisms and regulate the rate at which the chemical reactions takes place is known as an enzyme.

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Answer:

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It is significant since it enables you to decide on the material best  cost, construction, lifespan, and design.

Studying the properties of materials is essential when designing new products because it ensures that the materials used meet the necessary requirements for the product's intended function and performance. The properties of materials determine how they behave in different environment , and therefore understanding these properties is crucial to create safe, effective, and reliable products.

For example, if a product requires a material that is strong and durable, the designer needs to know the mechanical properties of different materials, such as tensile strength, compressive strength, and impact resistance, to choose the right material that can withstand the expected loads and stresses. If a product is expected to operate under different temperatures or environments, then the designer must know the thermal and chemical properties of different materials to select the most suitable one.

Moreover, different materials have different manufacturing requirements, and understanding their properties can help designers choose the most appropriate manufacturing processes and techniques for their products. For instance, the properties of materials, such as melting point, ductility, and hardness, affect the machining, casting, and forming processes that can be used to manufacture them.

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Final answer:

Studying the properties of materials when designing new products is crucial as it determines the product's performance, durability and safety. Understanding these properties can also lead to cost reductions, more efficient use of resources, and enhanced sustainability.

Explanation:

When designing new products, studying the properties of materials is crucial for several reasons. The performance, durability, and safety of a final product are directly related to the materials used in its construction.

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Answers

Answer:

Answer is D.

Explanation:

The first cleavage division can be explained or described as the series of first cellular division of the zygote after fertilization.

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Major survival surgery on non-rodent mammals

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Answers

Answer:

Plants and animals assimilate carbon-14 from carbon dioxide throughout their life. When they die, they stop exchanging carbon with the biosphere and their carbon-14 content begins to decrease at a rate determined by the law of radioactive decay.

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Explanation:

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