A section of a genome is cut with three enzymes: A, B, and C. Cutting with A and B yields a 10-kb fragment. Cutting with B and C yields a 2-kb fragment. What is the expected result from a digest with A and C, if the C site lies in between the A and B sites?

Answers

Answer 1
Answer:

Answer:

8-kb

Explanation:

As you can see in the picture attached, the cutting sites are consecutively situated in the genome. For that reason, if the largest fragment results when A and B cut, and the cutting site of C is betwen A and B, then the fragment expected from a digest with A and C could be calculated as follows:

AC=AB-BC =10 kb- 2kb=8kb


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Is global warming greater near the equator or near the poles

Answers

Answer:

The poles

Explanation:

Because the air is thinner and more sun radiation.

In the five-kingdom classification system, all prokaryotes belong to the kingdom Monera. Which of the following best explains a reason why prokaryotes were separated into different domains, Eubacteria and Archaea?

Answers

From a five kingdom classification system, it became a three domain classification system. Prokaryotes were separated into different domains because archaea merely resembled only bacteria morphologically but they are unicellular and have no organelles. Archaeans are also known to exist in extreme environmental conditions, which makes them different from Eubacteria.

Prokaryotes were initially grouped in the kingdom Monera, but they were eventually separated into different domains (Eubacteria and Archaea) based on evolutionary and genetic differences. This allows for a more accurate classification of prokaryotes.

In the five-kingdom classification system, prokaryotes were initially grouped together under the kingdom Monera because they share certain characteristics, such as lacking a nucleus and membrane-bound organelles. However, as scientific knowledge advanced, it was discovered that prokaryotes are actually divided into two distinct domains: Eubacteria and Archaea.

The separation of prokaryotes into different domains was based on evolutionary and genetic differences. For example, archaea have distinct genetic and biochemical features that set them apart from bacteria, such as their unique cell wall composition and ability to live in extreme environments.

So, the separation into domains allows for a more accurate classification and understanding of the diversity and complexity of prokaryotes.

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Different mRNA molecules can have poly-A tails of different lengths. Considering the purpose of adding the poly-A tail (from the previous question), why are some tails longer than others?

Answers

Answer:  Then the longer it is, the more it protects against degradation. Proteins that are needed over a long period of time come from a long tailed mRNA.

Explanation:

Messenger RNA (mRNA) is the ribonucleic acid that transfers the genetic code from the DNA of the cell nucleus to a ribosome in the cytoplasm. It determines the order in which the amino acids in a protein will bind and acts as a template or pattern for the synthesis of that protein.

Messenger RNA is synthesized in the cell nucleus in eukaryotes from the process called DNA transcription. In most cases, once this messenger RNA has been synthesized, it must be matured (RNA maturation). This involves the removal of intercalary sequences called non-coding introns from the protein to be synthesized. Then, the addition of a structure called CAP to the 5' end, which is a modified guanine nucleotide needed for the normal process of DNA transplantation and maintaining its stability. This is critical for proper recognition and access of the ribosome.  Also, polyadenylation which is the addition of the sequence called Poly-A to the 3' end. The Poly-A sequence is made up of several adenine molecules and is located at about 20-30 bp towards the tail (AAPAA sequence) or polyadenylation signal, which protects the end of the mRNA. The polyadenylation helps to increase the period of the message, so that the transcription lasts longer in the cell and therefore more protein is translated and produced.

This mature messenger RNA is transferred to the cell's cytoplasm, in the case of eukaryotes, through pores in the nuclear membrane.  The messenger RNA in the cytoplasm is coupled to the ribosomes, which are the machinery in charge of protein synthesis.  However, after a certain amount of time the mRNA is degraded into its component nucleotides, usually with the help of ribonucleases. So, an mRNA with a short tail will have a shorter lifespan.

Then, proteins that are needed over a long period of time come from a long tailed mRNA. And proteins that are briefly nedded come from a short tailed mRNA. This is because, as explained, the Poly-A tail serves to protect the mRNA molecule from degradation. So, then the longer it is, the more it protects against degradation.

A tiltmeter is a volcanic monitoring tool that measures _____.

Answers

A tiltmeter is a volcanic monitoring tool that measures ground swelling.

A tiltmeter is an instrument that is used to determine if the elevation of the earth crust has changed. Tiltmeters measures the angle of an object with regards to gravity. Tiltmeters are highly used in monitoring dams, volcanoes, hydraulic fractures and landslides.


A tiltmeter monitors the earth's crust to determine if any uplift is occuring. Simply put, it measures the crust to see if the elevation has changed.

Which cell cycle checkpoint determines whether the DNA has been replicated correctly? a. the cell growth checkpoint b. the DNA synthesis checkpoint c. the mitosis checkpoint d. the G2 checkpoint

Answers

b. The DNA synthesis checkpoint

A wilted flower placed in a vase of water for several hours became stiff and stood erect. When it was placed in a salt solution, it wilted. From this information what can we say about the cells of the flower? A) the flower cells are hypertonic to fresh water but hypotonic to the salt solution. B) the flower cells are hypotonic to the fresh water and hypertonic to the salt solution.C) the flower cells are hypertonic to both the fresh water and the salt solution.D) the flower cells are isotonic to both the fresh and salt water solutions

Answers

Answer:

Option A

Explanation:

The flower cells are hypertonic to fresh water but hypotonic to the salt solution.

Flower cell is hypertonic to fresh water, this means that the amount of solute in the solution outside the flower cell is higher than the solute content inside the flower cell.

While when a flower cell is hypotonic, it means that the amount of solute in the solution outside the flower cell is lower than the solute content inside the flower cell.

This is the reason why a flower plant placed in vase of water stood and become erect, while the same flower placed in a salt solution wilted.

Hence, option A is correct

Final answer:

The flower cells behave as hypertonic in fresh water, causing them to absorb water and stand erect, while they act as hypotonic in a salt solution, leading to water leaving the cells and wilting the flower.

Explanation:

From the information given about the flower's cells, the correct answer is A) the flower cells are hypertonic to fresh water but hypotonic to the salt solution. This is due to the process of osmosis, which is the movement of water across a semi-permeable membrane, like a cell wall, from areas of low solute concentration (hypotonic) to areas of high solute concentration (hypertonic).

When the wilted flower was placed in the fresh water, the cells were hypertonic causing water to enter the cells and making the flower stand erect. This is because there were more solutes inside the cells than the fresh water solution surrounding them.

On the other hand, when the flower was placed in the salt solution, it wilted because the cells were hypotonic compared to the hypertonic salt solution. There were fewer solutes in the flower cells than the salt solution outside, so water left the cells, which made the flower wilt.

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