Answer:
the a)
Explanation:
The molecules that have the different structural formula but the same chemical formula are known as isomers. Isomers explain the relationship between molecular arrangements that, although differing in chemical or physical properties. Chemical reactions in which one isomer is transformed into different are called isomerizations. A rapid equilibrium that cannot be easily isolated involved by isomerization is known as tautomerization.
B. magnified self-image
C. desire for beauty
D. mind-body conflict
Assaying the samples in triplicate is a way of control.
Using triplicates will allow you to account for variation within the assay (intra-assay variation). This means that if you do not get the same result in all triplicate wells, you probably have a problem with experimental technique or you have made a pipetting error, so the experiment should have be repeated.
Running an ELISA assay in triplicate increases the reliability and accuracy of results by providing a means of calculating the average from three measurements and mitigating the impact of random errors or procedural inconsistencies. Replicating the assay helps to ensure that results are robust and that variations in individual steps of an experiment do not significantly skew the data.
Completing an ELISA assay in triplicate helps to improve the accuracy of results by addressing statistical variability and potential procedural errors. Testing in triplicate ensures repeated measurements of the same sample, reducing the effect of random errors across the tests and thereby enhancing the reliability and consistency of the data collected.
In a situation where a scientist is measuring the amount of a specific protein in a blood sample, even slight inconsistencies in procedures could impact the results. For example, if the secondary antibody is not washed off thoroughly in one test, it might lead to a false increase in signal strength indicating a higher protein level than actually present. Running the assay in triplicates can mitigate this, as the outlier value can be spotted by comparing it against the other two measurements. It also allows the scientist to calculate the mean value of the three tests, providing a more robust estimation of the protein level. Thus, doing ELISA in triplicate better ensures that your results are reproducible and accurate.
Additionally, each ELISA reaction is a complex process involving a series of binding, washing, and coloring steps. Variations can creep in such as pipetting errors, uneven incubation temperatures, or variation in color development times. Hence reliable and repeatable results in scientific experiments often involve running multiple replicates, including in ELISA assays.
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