Why is it important to sequence your plasmid after purifying it?

Answers

Answer 1
Answer: Here is my suggestion. ALWAYS you digest a vector you should do a gel-purification step. Some people will say that they don't do it because it's a waste of time and that is ok, however, if you do it you are practicing good molecular biology as defined by Sambrook&Maniatis, and these guys knew something about it. Therefore, I will encourage you to do this step as often as you can, this will ensure you have no traces of undigested vector that will interfere during your transformation step (e.g. false positive) and consequently save you time later. The best way to save time is to do it right. You also want to get rid of buffers and ions that may (or may not) interfere with your phosphatase steps and have a clear start too. The later is not so critical though, as most reagents are now quite compatible with each other as well as between companies, which is great. Then, after phosphatase treatment the cleaning step depends on two things: if you used CIP (NEB), this enzyme you MUST gel-purify (recommended) or use a kit to get rid of the CIP that tightly binds to the end of your DNA and cannot be heat inactivated. If you used TSAP (Promega) 0r any other thermosensitive phosphatase, then you can heat inactivate it. The next thing to take into account is to check the buffer compatibilities between your recently 'phosphatased' vector, your digested construct and your ligation buffer. Most of the T4 DNA ligase in the market are compatible with any of the buffers for restrictions and phosphatase. For your Insert in the experimental setting you have provided, I believe you can skip it.

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An upper respiratory tract infection will primarily affect what sense?

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An upper respiratory tract infection will primarily affect the sense of smell. It occurs during the common cold when you lose the sense of smell due to problems with the upper respiratory tract, that is, your nose gets stuffed.

In humans, the hormone testosterone enters cells and binds to specific proteins, which in turn bind to specific sites on the cells' DNA. These proteins probably act to do what? See Concept 18.2

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Answer:

Translation may be defined as the formation of the protein product from the RNA molecule. Different enzymes and proteins are required for the process of translation. Translation involves three steps - initiation, elongation and termination.

The testosterone hormone in humans helps in the development of the male secondary sexual characters. The testosterone hormone binds with the proteins and then protein binds to the specific DNA sites. These proteins helps in the transcription of the certain genes and may acts as the enhancers.

One parent has curly hair, the other has straight hair, and their child has wavy hair. This is an example ofA. incomplete dominance.
B. polygenic inheritance.
C. codominance.
D. multiple allele traits.

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Answer: The correct answer is- A) Incomplete dominance.

Incomplete dominance means neither of the allele ( that is dominant and recessive allele) shows its phenotype complelety. In other words, no allele is completely dominant and a heterozygous condition produces an intermediate phenotype that lies between a dominant and a recessive trait.

As per the question, the phenotype of parents are - curly hair and straight hair.

As child expresses and intermediate phenotype that is wavy hair, therefore it is an example of incomplete dominance.      

Incomplete dominance

List negative effects of the use of chemical fertilizers

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It destroys the quality of soil
it kills the useful micro organisms present in the soil

The development of the cell theory involved ___________________________. a. different ideas from different scientists.
b. the efforts of one scientist.
c. work with computer models.
d. complete agreement among scientists.

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The answer is D. It took different scientists's inventions/discoveries to come to a conclusion which was the cell theory. They didn't have computers before they developed the cell theory so, it's not c.

The answer is a different ideas from different scientist.


Hope this helps!

The structure of adenosine triphosphate (ATP) is shown here. Which statement best explains how energy stored in the chemical bonds of ATP can be released. A) Adenine is removed by hydrolysis from an ATP molecule. B) Electrons are removed by hydrolysis from an ATP molecule. C) A phosphate is removed by hydrolysis from an ATP molecule. D) A phosphate group is added to an ATP molecule with the addition of H2O.

Answers

C) A phosphate is removed by hydrolysis from an ATP molecule.

* ATP contains Adenine, Ribose sugar, and triphosphate group that contains 3 phosphates. When the third phosphate is broken, it is converted to ADP + P releasing a lot of energy.

The phosphate is removed by hydrolysis from ATP molecules. Thus, energy is stored in the chemical bonds of ATP and released during ATP hydrolysis.

Further Explanation:

Adenosine triphosphate (ATP) is the molecular currency used for energy transactions. The ATP energy is stored in the bonds between the terminal phosphate groups. These bonds are hydrolyzed by the enzymes to obtain energy for reactions. ATP is an energy caring molecule present in the living cell. It stores energy in the bonds between the phosphates produced by the breakdown of food molecules. It releases energy required for the chemical reaction, and hence, it is known as an effective energy donor.

ATP is made of three main structures:

  • Sugar (ribose)
  • Nitrogenous base (Adenine)
  • Three phosphate group

The main energy source in ATP is phosphate tail. The energy is stored in the bonds present between the phosphates groups and release when these bonds are broke down. This process is referred to as ATP hydrolysis in this addition of water occurs. When the one phosphate is removed from ATP, then it is converted into ADP.

Learn more:

  1. Learn more about cellular respiration brainly.com/question/543244
  2. Learn more about diffusion brainly.com/question/1386629
  3. Learn more about phospholipids bilayer brainly.com/question/751529

Answer Details:

Grade: High School

Subject: Biology

Chapter: Membrane Biology

Keywords:

ATP, enzymes, bonds, chemical reaction, energy, phosphate group, ADP, food molecule, breakdown, donor, sugar, ribose.